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What are the caveats of using erlenmeyer conical flask to ensure the accuracy ofthe experiment?

1.Speed and angle of shaking

The speed and anale of the shaking bottle is one of important factors that affect the results of the experimentIf the shaking is too fast or too slow, it may affect the growth of the cells and the mixing of the culture solution.The angle ofthe shaking needs to be adjusted based on the needs ofthe experiment to ensure that the culture solution can fuy contact the cellswhen shaking.

2. Time and frequency

The time and frequency of shaking are also important points to note.

If shaking too short, the cuiture solution may not mix adequately, while shaking too long may cause overly vigorous cell movement, which may affeclthe growth and health of the cells.

The frequency of shaking also needs to be adjusted any time to ensure the culture solution can be mixed continuously.

3.Temperature and humidity control

Temperature and humidity are also important during the shaking process

Too high or too low temperature may affect the growth of cells and the stability of the culture solution.

Excessive humidity may lead to evaporation of the culture medium and loss of water from the cells.